The routine preparation of diphtheria toxin

Preparation of a low-chloride Medium, for the production of Diphtheria toxin

The Routine Preparation of Scarlet-fever Toxin of High Value

A NUMBER of attempts have been made in recent years to improve the quality of scarlet fever toxin, of which the most noteworthy is that of Hooker et al. (1933), who by a special technique obtained toxin with a value of 500,000 skin test doses per c.c. For some time past, however, the routine toxins prepared in this laboratory have been of considerably greater potency than those described by the...

The Production of Diphtheria Toxin

Toxin of sufficient strength to kill a 400-gramme guinea-pig in three days and a half in a dose of 0.cubic centimetre developed in suitable bouillon, contained in ordinary Erlenmeyer flasks, within a period of twenty-four hours. In such boullon the toxin reached its greatest strength in from four to seven days (0.005 cubic centimetre killing a 500-gramme guinea-pig in three days). This period o...

preparation of a low-chloride medium, for the production of diphtheria toxin

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Diphtheria Toxin In

Recent studies have demonstrated that diphtheria toxin is an enzyme of unusual type. Small amounts of the toxin, added to nicotinamide adenine dinucleotide (NAD)-containing mammalian cell extracts, block peptide-bond formation by catalyzing inactivation of the translocating enzyme, aminoacyltransferase 2 (T2) (1-3). This highly specific reaction involves the splitting of NAD with liberation of ...